BomMDH1 regulates malate-mediated oxidative stress in tobacco BY-2 suspension cells

Copyright © 2024 Elsevier GmbH. All rights reserved.

Détails bibliographiques
Publié dans:Journal of plant physiology. - 1979. - 300(2024) vom: 01. Sept., Seite 154297
Auteur principal: Chen, Haohui (Auteur)
Autres auteurs: Cao, Shifeng, Chen, Jiahui, Wang, Hongfei, Wei, Yingying, Chen, Yi, Shao, Xingfeng, Xu, Feng
Format: Article en ligne
Langue:English
Publié: 2024
Accès à la collection:Journal of plant physiology
Sujets:Journal Article BY-2 suspension cells BomMDH1 Malate Oxidative stress PCD SHAM Malates malic acid 817L1N4CKP plus... Reactive Oxygen Species Hydrogen Peroxide BBX060AN9V Plant Proteins Malate Dehydrogenase EC 1.1.1.37 Malondialdehyde 4Y8F71G49Q
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520 |a Programmed cell death (PCD) is a genetically regulated process of cell suicide essential for plant development. The 'malate valve' is a mechanism that ensures redox balance across different subcellular compartments. In broccoli, the BomMDH1 gene encodes malate dehydrogenase in mitochondria, a critical enzyme in the 'malate circulation' pathway. This study investigates the functional role of BomMDH1 in malate (MA)-induced apoptosis in bright yellow-2 (BY-2) suspension cells. Findings revealed that transgenic cells overexpressing BomMDH1 showed enhanced viability under MA-induced oxidative stress compared to wild-type (WT) cells. Overexpression of BomMDH1 also reduced levels of reactive oxygen species (ROS), hydrogen peroxide (H2O2), and malondialdehyde (MDA), while increasing the expression of antioxidant enzyme genes such as NtAPX, NtAOX1a, NtSOD, and NtMDHAR. Additionally, treatment with salicylhydroxamic acid (SHAM), a characteristic inhibitor of mitochondrial respiration, further improved the anti-apoptotic activity of BY-2 cells. Overall, these results highlighted the function of the BomMDH1 gene and the potential of SHAM treatment in mitigating oxidative stress in BY-2 suspension cells 
650 4 |a Journal Article 
650 4 |a BY-2 suspension cells 
650 4 |a BomMDH1 
650 4 |a Malate 
650 4 |a Oxidative stress 
650 4 |a PCD 
650 4 |a SHAM 
650 7 |a Malates  |2 NLM 
650 7 |a malic acid  |2 NLM 
650 7 |a 817L1N4CKP  |2 NLM 
650 7 |a Reactive Oxygen Species  |2 NLM 
650 7 |a Hydrogen Peroxide  |2 NLM 
650 7 |a BBX060AN9V  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Malate Dehydrogenase  |2 NLM 
650 7 |a EC 1.1.1.37  |2 NLM 
650 7 |a Malondialdehyde  |2 NLM 
650 7 |a 4Y8F71G49Q  |2 NLM 
700 1 |a Cao, Shifeng  |e verfasserin  |4 aut 
700 1 |a Chen, Jiahui  |e verfasserin  |4 aut 
700 1 |a Wang, Hongfei  |e verfasserin  |4 aut 
700 1 |a Wei, Yingying  |e verfasserin  |4 aut 
700 1 |a Chen, Yi  |e verfasserin  |4 aut 
700 1 |a Shao, Xingfeng  |e verfasserin  |4 aut 
700 1 |a Xu, Feng  |e verfasserin  |4 aut 
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