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240701s2024 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2024.154297
|2 doi
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|a pubmed25n1247.xml
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|a (DE-627)NLM374329494
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|a (NLM)38945071
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|a (PII)S0176-1617(24)00128-7
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Chen, Haohui
|e verfasserin
|4 aut
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| 245 |
1 |
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|a BomMDH1 regulates malate-mediated oxidative stress in tobacco BY-2 suspension cells
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 08.07.2024
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|a Date Revised 02.08.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2024 Elsevier GmbH. All rights reserved.
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|a Programmed cell death (PCD) is a genetically regulated process of cell suicide essential for plant development. The 'malate valve' is a mechanism that ensures redox balance across different subcellular compartments. In broccoli, the BomMDH1 gene encodes malate dehydrogenase in mitochondria, a critical enzyme in the 'malate circulation' pathway. This study investigates the functional role of BomMDH1 in malate (MA)-induced apoptosis in bright yellow-2 (BY-2) suspension cells. Findings revealed that transgenic cells overexpressing BomMDH1 showed enhanced viability under MA-induced oxidative stress compared to wild-type (WT) cells. Overexpression of BomMDH1 also reduced levels of reactive oxygen species (ROS), hydrogen peroxide (H2O2), and malondialdehyde (MDA), while increasing the expression of antioxidant enzyme genes such as NtAPX, NtAOX1a, NtSOD, and NtMDHAR. Additionally, treatment with salicylhydroxamic acid (SHAM), a characteristic inhibitor of mitochondrial respiration, further improved the anti-apoptotic activity of BY-2 cells. Overall, these results highlighted the function of the BomMDH1 gene and the potential of SHAM treatment in mitigating oxidative stress in BY-2 suspension cells
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|a Journal Article
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|a BY-2 suspension cells
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|a BomMDH1
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|a Malate
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|a Oxidative stress
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|a PCD
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|a SHAM
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|a Malates
|2 NLM
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|a malic acid
|2 NLM
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| 650 |
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|a 817L1N4CKP
|2 NLM
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|a Reactive Oxygen Species
|2 NLM
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|a Hydrogen Peroxide
|2 NLM
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| 650 |
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|a BBX060AN9V
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Malate Dehydrogenase
|2 NLM
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| 650 |
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|a EC 1.1.1.37
|2 NLM
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| 650 |
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|a Malondialdehyde
|2 NLM
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| 650 |
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|a 4Y8F71G49Q
|2 NLM
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| 700 |
1 |
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|a Cao, Shifeng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Jiahui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Hongfei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wei, Yingying
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Yi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shao, Xingfeng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xu, Feng
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 300(2024) vom: 01. Sept., Seite 154297
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnas
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| 773 |
1 |
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|g volume:300
|g year:2024
|g day:01
|g month:09
|g pages:154297
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| 856 |
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|u http://dx.doi.org/10.1016/j.jplph.2024.154297
|3 Volltext
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