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240429s2024 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2024.108663
|2 doi
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|a pubmed24n1453.xml
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|a (DE-627)NLM371677939
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|a (NLM)38678947
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|a (PII)S0981-9428(24)00331-0
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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|a Qiao, Qinghai
|e verfasserin
|4 aut
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|a The PbbHLH62/PbVHA-B1 module confers salt tolerance through modulating intracellular Na+/K+ homeostasis and reactive oxygen species removal in pear
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 09.05.2024
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|a Date Revised 26.06.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2024 Elsevier Masson SAS. All rights reserved.
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|a The vacuolar H+-ATPase (V-ATPase) is a multi-subunit membrane protein complex, which plays pivotal roles in building up an electrochemical H+-gradient across tonoplast, energizing Na+ sequestration into the central vacuole, and enhancing salt stress tolerance in plants. In this study, a B subunit of V-ATPase gene, PbVHA-B1 was discovered and isolated from stress-induced P. betulaefolia combining with RT-PCR method. The RT-qPCR analysis revealed that the expression level of PbVHA-B1 was upregulated by salt, drought, cold, and exogenous ABA treatment. Subcellular localization analyses showed that PbVHA-B1 was located in the cytoplasm and nucleus. Moreover, overexpression of PbVHA-B1 gene noticeably increased the ATPase activity and the tolerance to salt in transgenic Arabidopsis plants. In contrast, knockdown of PbVHA-B1 gene in P.betulaefolia by virus-induced gene silencing had reduced resistance to salt stress. In addition, using yeast one-hybride (Y1H) and yeast two-hybride (Y2H) screens, PbbHLH62, a bHLH transcription factor, was identified as a partner of the PbVHA-B1 promoter and protein. Then, we also found that PbbHLH62 positively regulate the expression of PbVHA-B1 and the ATPase activity after salt stress treatment. These findings provide evidence that PbbHLH62 played a critical role in the salt response. Collectively, our results demonstrate that a PbbHLH62/PbVHA-B1 module plays a positive role in salt tolerance by maintain intracellular ion and ROS homeostasis in pear
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|a Journal Article
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|a Ion and reactive oxygen species homeostasis
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|a P.betulaefolia
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|a PbVHA-B1
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|a PbbHLH62
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|a Salt tolerance
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|a V-ATPase
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|a Plant Proteins
|2 NLM
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|a Reactive Oxygen Species
|2 NLM
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|a Sodium
|2 NLM
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| 650 |
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|a 9NEZ333N27
|2 NLM
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|a Potassium
|2 NLM
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|a RWP5GA015D
|2 NLM
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|a Vacuolar Proton-Translocating ATPases
|2 NLM
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|a EC 3.6.1.-
|2 NLM
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| 700 |
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|a Huang, Yongdan
|e verfasserin
|4 aut
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|a Dong, Huizhen
|e verfasserin
|4 aut
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|a Xing, Caihua
|e verfasserin
|4 aut
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|a Han, Chenyang
|e verfasserin
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|a Lin, Likun
|e verfasserin
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|a Wang, Xin
|e verfasserin
|4 aut
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|a Su, Zhiyuan
|e verfasserin
|4 aut
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|a Qi, Kaijie
|e verfasserin
|4 aut
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|a Xie, Zhihua
|e verfasserin
|4 aut
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|a Huang, Xiaosan
|e verfasserin
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|a Zhang, Shaoling
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 210(2024) vom: 26. Mai, Seite 108663
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:210
|g year:2024
|g day:26
|g month:05
|g pages:108663
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|u http://dx.doi.org/10.1016/j.plaphy.2024.108663
|3 Volltext
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