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240114s2024 xx |||||o 00| ||eng c |
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|a 10.1111/jfd.13922
|2 doi
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|a pubmed25n1222.xml
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|a (DE-627)NLM366947834
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|a eng
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| 100 |
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|a He, Huanrong
|e verfasserin
|4 aut
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|a A novel defensin-like peptide C-13326 possesses protective effect against multidrug-resistant Aeromonas schubertii in hybrid snakehead (Channa maculate ♀ × Channa argus ♂)
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|c 2024
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 11.03.2024
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|a Date Revised 11.03.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2024 John Wiley & Sons Ltd.
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|a The purpose of this study was to investigate whether a defensin-like antimicrobial peptide (C-13326 peptide) identified in Hermetia illucens could possess protective effect against multidrug-resistant Aeromonas schubertii in hybrid snakehead (Channa maculate ♀ × Channa argus ♂). The cDNA of C-13326 peptide comprised 243 nucleotides encoding 80 amino acids, with six conserved cysteine residues and the classical CSαβ structure. The recombinant expression plasmid pPIC9K-C-13326 was constructed and transformed into GS115 Pichia pastoris, and the C-13326 peptide was expressed by induction with 1% methanol. The crude extract of C-13326 peptide was precipitated by ammonium sulfate, assayed by Braford method, detected by tricine-SDS-PAGE, evaluated by BandScan software and identified by liquid chromatography-mass spectrometry. The C-13326 peptide was shown to have inhibitory activity against the growth of multidrug-resistant A. schubertii DM210910 by using the minimum growth inhibitory concentration and Oxford cup method. In addition, scanning electron microscopy analysis suggested that C-13326 peptide inhibited the growth of A. schubertii DM210910 by damaging the bacterial cell membrane. To explore the role of peptide C-13326 in vivo, hybrid snakehead was fed with peptide C-13326 as feed additives for 7 days. The results revealed that C-13326 peptide could significantly down-regulate the expression levels of IL-1β, IL-8, IL-12 and TNF-α (p < .05), and significantly improved the survival rate of hybrid snakehead after challenging with A. schubertii DM210910. Therefore, the C-13326 peptide is a promising antimicrobial agent for A. schubertii treatment in aquaculture
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|a Journal Article
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|a Aeromonas schubertii
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|a black soldier fly
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|a defensins
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4 |
|a hybrid snakehead
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|a polypeptide C
|2 NLM
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|a Peptides
|2 NLM
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| 650 |
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|a Defensins
|2 NLM
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| 700 |
1 |
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|a Huang, Xinzhi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wen, Caiyi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Chun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jiang, Biao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Huang, Yanhua
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Su, Youlu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Wei
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 47(2024), 4 vom: 03. Apr., Seite e13922
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnas
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| 773 |
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|g volume:47
|g year:2024
|g number:4
|g day:03
|g month:04
|g pages:e13922
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| 856 |
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|u http://dx.doi.org/10.1111/jfd.13922
|3 Volltext
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