Reference genes for gene expression profiling in mouse models of Listeria monocytogenes infection
RT-qPCR dissects transcription-based processes but requires reference genes (RGs) for data normalization. This study prospected RGs for mouse macrophages (pMØ) and spleen infected with Listeria monocytogenes. The pMØ were infected in vitro with L. monocytogenes or vehicle for 4 h. Mice were injected...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 76(2024), 3 vom: 09. März, Seite 104-113
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| 1. Verfasser: |
Tavares, Lethicia Souza
(VerfasserIn) |
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| Weitere Verfasser: |
Oliveira-Silva, Roberta Lane,
Moura, Marcelo Tigre,
da Silva, Jéssica Barboza,
Benko-Iseppon, Ana Maria,
Lima-Filho, José Vitor |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2024
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
RT-qPCR
cytokine
housekeeping genes
immune response
internal control genes
listeriosis
macrophages
real-time qPCR
relative expression
mehr...
spleen
Cytokines |
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