The multi-omics single-cell landscape of sinus mucosa in uncontrolled severe chronic rhinosinusitis with nasal polyps

The multi-omics single-cell landscape of sinus mucosa in uncontrolled severe chronic rhinosinusitis with nasal polyps

Copyright © 2023 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 256(2023) vom: 31. Nov., Seite 109791
1. Verfasser: Xu, Zhaofeng (VerfasserIn)
Weitere Verfasser: Huang, Yanran, Meese, Tim, Van Nevel, Sharon, Holtappels, Gabriele, Vanhee, Stijn, Bröker, Barbara M, Li, Zhengqi, de Meester, Ellen, De Ruyck, Natalie, Van Zele, Thibaut, Gevaert, Philip, Van Nieuwerburgh, Filip, Zhang, Luo, Shamji, Mohamed H, Wen, Weiping, Zhang, Nan, Bachert, Claus
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Chronic rhinosinusitis with nasal polyps Immune profiles Tissue remodeling Type 2 immune responses scRNA-seq Receptors, Antigen, B-Cell
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520 |a Uncontrolled severe chronic rhinosinusitis with nasal polyps (CRSwNP) is associated with elevated levels of type 2 inflammatory cytokines and raised immunoglobulin concentrations in nasal polyp tissue. By using single-cell RNA sequencing, transcriptomics, surface proteomics, and T cell and B cell receptor sequencing, we found the predominant cell types in nasal polyps were shifted from epithelial and mesenchymal cells to inflammatory cells compared to nasal mucosa from healthy controls. Broad expansions of CD4 T effector memory cells, CD4 tissue-resident memory T cells, CD8 T effector memory cells and all subtypes of B cells in nasal polyp tissues. The T and B cell receptor repertoires were skewed in NP. This study highlights the deviated immune response and remodeling mechanisms that contribute to the pathogenesis of uncontrolled severe CRSwNP. CLINICAL IMPLICATIONS: We identified differences in the cellular compositions, transcriptomes, proteomes, and deviations in the immune profiles of T cell and B cell receptors as well as alterations in the intercellular communications in uncontrolled severe CRSwNP patients versus healthy controls, which might help to define potential therapeutic targets in the future 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Chronic rhinosinusitis with nasal polyps 
650 4 |a Immune profiles 
650 4 |a Tissue remodeling 
650 4 |a Type 2 immune responses 
650 4 |a scRNA-seq 
650 7 |a Receptors, Antigen, B-Cell  |2 NLM 
700 1 |a Huang, Yanran  |e verfasserin  |4 aut 
700 1 |a Meese, Tim  |e verfasserin  |4 aut 
700 1 |a Van Nevel, Sharon  |e verfasserin  |4 aut 
700 1 |a Holtappels, Gabriele  |e verfasserin  |4 aut 
700 1 |a Vanhee, Stijn  |e verfasserin  |4 aut 
700 1 |a Bröker, Barbara M  |e verfasserin  |4 aut 
700 1 |a Li, Zhengqi  |e verfasserin  |4 aut 
700 1 |a de Meester, Ellen  |e verfasserin  |4 aut 
700 1 |a De Ruyck, Natalie  |e verfasserin  |4 aut 
700 1 |a Van Zele, Thibaut  |e verfasserin  |4 aut 
700 1 |a Gevaert, Philip  |e verfasserin  |4 aut 
700 1 |a Van Nieuwerburgh, Filip  |e verfasserin  |4 aut 
700 1 |a Zhang, Luo  |e verfasserin  |4 aut 
700 1 |a Shamji, Mohamed H  |e verfasserin  |4 aut 
700 1 |a Wen, Weiping  |e verfasserin  |4 aut 
700 1 |a Zhang, Nan  |e verfasserin  |4 aut 
700 1 |a Bachert, Claus  |e verfasserin  |4 aut 
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773 1 8 |g volume:256  |g year:2023  |g day:31  |g month:11  |g pages:109791 
856 4 0 |u http://dx.doi.org/10.1016/j.clim.2023.109791  |3 Volltext 
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