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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1111/jfd.13675
|2 doi
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|a pubmed24n1142.xml
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Schuster, Corbin J
|e verfasserin
|4 aut
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| 245 |
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|a Progression of infection and detection of Pseudoloma neurophilia in zebrafish Danio rerio Hamilton by PCR and histology
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|c 2022
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|a Text
|b txt
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 15.09.2022
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|a Date Revised 15.09.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2022 John Wiley & Sons Ltd.
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|a Pseudoloma neurophilia is a critical threat to the zebrafish (Danio rerio) model, as it is the most common infectious agent found in research facilities. In this study, our objectives were two-fold: (1) compare the application of diagnostic tools for P. neurophilia and (2) track the progression of infection using PCR and histology. The first experiment showed that whole-body analysis by qPCR (WB-qPCR) can be a standardized process, providing a streamlined diagnostic protocol, without the need for extraction of specific tissues. Evaluating the course of infection in experimentally infected fish, we showed key dynamics in infection. Starting with a low dose exposure of 8000 spores/fish, the prevalence remained low until 92 days post-exposure (dpe), followed by a 30%-40% prevalence by histology or 40%-90% by PCR until the end of the experiment at 334 dpe. WB-qPCR positively detected infection in more fish than histology throughout the study, as WB-qPCR detected the parasite as early as 4 dpe, whereas it was undetected by histology until 92 dpe. We also added a second slide for histologic analyses, showing an increase in detection rate from 24% to 26% when we combined all data from our experiments, but this increase was not statistically significant
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|a Journal Article
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|a diagnostics
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|a microsporidia
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|a zebrafish
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|a Kreul, Taylor G
|e verfasserin
|4 aut
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|a Al-Samarrie, Colleen E
|e verfasserin
|4 aut
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| 700 |
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|a Peterson, James T
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sanders, Justin L
|e verfasserin
|4 aut
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| 700 |
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|a Kent, Michael L
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 45(2022), 10 vom: 01. Okt., Seite 1463-1475
|w (DE-627)NLM098166034
|x 1365-2761
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|g volume:45
|g year:2022
|g number:10
|g day:01
|g month:10
|g pages:1463-1475
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|u http://dx.doi.org/10.1111/jfd.13675
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