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231226s2022 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202200653
|2 doi
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|a pubmed25n1137.xml
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|a (DE-627)NLM341182982
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|a (NLM)35595711
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|a DE-627
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|e rakwb
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|a eng
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|a Ryma, Matthias
|e verfasserin
|4 aut
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|a A Print-and-Fuse Strategy for Sacrificial Filaments Enables Biomimetically Structured Perfusable Microvascular Networks with Functional Endothelium Inside 3D Hydrogels
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 15.07.2022
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|a Date Revised 15.07.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2022 The Authors. Advanced Materials published by Wiley-VCH GmbH.
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|a A facile and flexible approach for the integration of biomimetically branched microvasculature within bulk hydrogels is presented. For this, sacrificial scaffolds of thermoresponsive poly(2-cyclopropyl-2-oxazoline) (PcycloPrOx) are created using melt electrowriting (MEW) in an optimized and predictable way and subsequently placed into a customized bioreactor system, which is then filled with a hydrogel precursor solution. The aqueous environment above the lower critical solution temperature (LCST) of PcycloPrOx at 25 °C swells the polymer without dissolving it, resulting in fusion of filaments that are deposited onto each other (print-and-fuse approach). Accordingly, an adequate printing pathway design results in generating physiological-like branchings and channel volumes that approximate Murray's law in the geometrical ratio between parent and daughter vessels. After gel formation, a temperature decrease below the LCST produces interconnected microchannels with distinct inlet and outlet regions. Initial placement of the sacrificial scaffolds in the bioreactors in a pre-defined manner directly yields perfusable structures via leakage-free fluid connections in a reproducible one-step procedure. Using this approach, rapid formation of a tight and biologically functional endothelial layer, as assessed not only through fluorescent dye diffusion, but also by tumor necrosis factor alpha (TNF-α) stimulation, is obtained within three days
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|a Journal Article
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|a bifurcating microchannels
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|a bioreactors
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|a endothelial function
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|a melt electrowriting
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|a poly(2-oxazoline)
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|a Hydrogels
|2 NLM
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|a Genç, Hatice
|e verfasserin
|4 aut
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|a Nadernezhad, Ali
|e verfasserin
|4 aut
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| 700 |
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|a Paulus, Ilona
|e verfasserin
|4 aut
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| 700 |
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|a Schneidereit, Dominik
|e verfasserin
|4 aut
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| 700 |
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|a Friedrich, Oliver
|e verfasserin
|4 aut
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| 700 |
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|a Andelovic, Kristina
|e verfasserin
|4 aut
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| 700 |
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|a Lyer, Stefan
|e verfasserin
|4 aut
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|a Alexiou, Christoph
|e verfasserin
|4 aut
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|a Cicha, Iwona
|e verfasserin
|4 aut
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|a Groll, Jürgen
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 34(2022), 28 vom: 01. Juli, Seite e2200653
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnas
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| 773 |
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|g volume:34
|g year:2022
|g number:28
|g day:01
|g month:07
|g pages:e2200653
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|u http://dx.doi.org/10.1002/adma.202200653
|3 Volltext
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