Extraction of cucumber phloem sap based on the capillary-air pressure principle
Changes in the substances in phloem sap can effectively reflect the nutritional status of cucumber plants during their growth. Because of the limitations of the time-consuming and complex operations of existing phloem sap extraction methods, the authors proposed a new extraction method based on the...
| Publié dans: | BioTechniques. - 1991. - 72(2022), 6 vom: 13. Juni, Seite 233-243 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2022
|
| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't EDTA GC-MS capillary glass tube capillary pressure method phloem sap Edetic Acid 9G34HU7RV0 |
| Résumé: | Changes in the substances in phloem sap can effectively reflect the nutritional status of cucumber plants during their growth. Because of the limitations of the time-consuming and complex operations of existing phloem sap extraction methods, the authors proposed a new extraction method based on the capillary-air pressure principle and designed a new sap sampling device. To examine the feasibility of the new sampling device, sap sampled from the same plant with the new method and the common EDTA method was analyzed by gas-phase mass spectrometry. The data showed that the number of substances in the sap sampled using capillary-air pressure was higher than that observed using the EDTA method. The concentration of substances sampled using capillary-air pressure was much higher than that observed using EDTA |
|---|---|
| Description: | Date Completed 16.06.2022 Date Revised 06.07.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1940-9818 |
| DOI: | 10.2144/btn-2021-0101 |