Selection and validation of reference genes for quantitative real-time PCR in Cymbidium sinense

Selection of reference genes (RGs) is important for the accurate analysis of real-time quantitative PCR (RT-qPCR) results. This study screened RGs of Cymbidium sinense for more accurate quantification of target genes. The two most stable RGs for all tissues were ACT and EF1α, those for vegetative or...

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Publié dans:BioTechniques. - 1991. - 72(2022), 2 vom: 16. Feb., Seite 51-59
Auteur principal: Tian, Yunfang (Auteur)
Autres auteurs: Chu, Zhigang, Wang, Huiyu, Wang, Guoxia, Wu, Si, Yang, Yuzhen
Format: Article en ligne
Langue:English
Publié: 2022
Accès à la collection:BioTechniques
Sujets:Journal Article Research Support, Non-U.S. Gov't Cymbidium sinense real-time fluorescent quantitative PCR reference genes