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231225s2022 xx |||||o 00| ||eng c |
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|a 10.2144/btn-2021-0093
|2 doi
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|a pubmed25n1112.xml
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Hanyu, Yoshiro
|e verfasserin
|4 aut
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| 245 |
1 |
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|a High-yield expression of periplasmic single-chain variable fragments by solid Escherichia coli cultures
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|c 2022
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 02.05.2022
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|a Date Revised 02.05.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a High-yield expression of quality antibody fragments is indispensable for research and diagnosis. Most recombinant antibody fragments are expressed in Escherichia coli using liquid cultures; however, their yields and quality are often poor. Here the authors expressed a single-chain variable fragment in E. coli cultivated on the wet surface of a solid support. Compared with a liquid culture, the authors obtained 2.5-times more single-chain variable fragments with membrane-cultivated E. coli. This method has two important advantages: it enables high yields of periplasmic single-chain variable fragments compared with liquid culture and offers simple and rapid expression and extraction
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4 |
|a Journal Article
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|a E. coli
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|a antibody
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|a expression
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4 |
|a periplasm
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| 650 |
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4 |
|a scFv
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| 650 |
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4 |
|a solid culture
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| 650 |
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|a Escherichia coli Proteins
|2 NLM
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| 650 |
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7 |
|a Recombinant Proteins
|2 NLM
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| 650 |
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7 |
|a Single-Chain Antibodies
|2 NLM
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| 700 |
1 |
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|a Kato, Mieko
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 72(2022), 1 vom: 29. Jan., Seite 29-32
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnas
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| 773 |
1 |
8 |
|g volume:72
|g year:2022
|g number:1
|g day:29
|g month:01
|g pages:29-32
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| 856 |
4 |
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|u http://dx.doi.org/10.2144/btn-2021-0093
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