PEG-modified subunit vaccine encoding dominant epitope to enhance immune response against spring viraemia of carp virus

© 2021 John Wiley & Sons Ltd.

Bibliographische Detailangaben
Veröffentlicht in:Journal of fish diseases. - 1998. - 44(2021), 10 vom: 24. Okt., Seite 1587-1594
1. Verfasser: Duan, Hui-Xin (VerfasserIn)
Weitere Verfasser: Zhao, Zhao, Jin, Ying-Jie, Wang, Zi-Long, Deng, Jie-Fang, He, Jie, Zhu, Bin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2021
Zugriff auf das übergeordnete Werk:Journal of fish diseases
Schlagworte:Journal Article Randomized Controlled Trial, Veterinary PEG common carps dominant epitopes immune response spring viraemia of carp virus Epitopes Vaccines, Subunit Viral Vaccines
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520 |a Spring viraemia of carp (SVC) caused by spring viraemia of carp virus (SVCV) can infect almost all fish of cyprinids, which bring huge economic losses to aquaculture. Glycoprotein (G), as the most important antigenic determinant protein of SVCV, is widely considered as an effective method against SVCV. In our previous study, we found that G3 (131 aa) is the potential dominant antigen epitope that induces strong immune responses similar to G protein (510 aa). Here, in order to further improve the immune effect, we reported a subunit vaccine (PEG-G3) constructed by PEG-modified dominant epitope protein (G3). The results of serum antibody production, enzyme activities and immune-related genes expression showed that PEG-G3 induces significantly stronger immune protective responses against SVCV than G3. PEG modification significantly increased the serum antibody level of the vaccine, which increased significantly after immunization and reached the peak at 21 day post-vaccination. T-AOC and AKP activities in the lowest concentration group (5 μg) of PEG-G3 were significantly higher than those in the highest concentration group (20 μg) of G3. In PEG-G3 group, the expression of almost all genes increased at least 4 times compared with the control group. After 14-day challenge, the RPS (relative percentage survival) of the highest concentration of PEG-G3 group was 53.6%, while that of G3 group is 38.9%. Therefore, this work shows that PEG modification and dominant epitope screening may be effective methods to improve the immune protective effect of vaccines and to resist the infection of aquatic animal viral diseases 
650 4 |a Journal Article 
650 4 |a Randomized Controlled Trial, Veterinary 
650 4 |a PEG 
650 4 |a common carps 
650 4 |a dominant epitopes 
650 4 |a immune response 
650 4 |a spring viraemia of carp virus 
650 7 |a Epitopes  |2 NLM 
650 7 |a Vaccines, Subunit  |2 NLM 
650 7 |a Viral Vaccines  |2 NLM 
700 1 |a Zhao, Zhao  |e verfasserin  |4 aut 
700 1 |a Jin, Ying-Jie  |e verfasserin  |4 aut 
700 1 |a Wang, Zi-Long  |e verfasserin  |4 aut 
700 1 |a Deng, Jie-Fang  |e verfasserin  |4 aut 
700 1 |a He, Jie  |e verfasserin  |4 aut 
700 1 |a Zhu, Bin  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Journal of fish diseases  |d 1998  |g 44(2021), 10 vom: 24. Okt., Seite 1587-1594  |w (DE-627)NLM098166034  |x 1365-2761  |7 nnns 
773 1 8 |g volume:44  |g year:2021  |g number:10  |g day:24  |g month:10  |g pages:1587-1594 
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