An assay of human tyrosine protein kinase ABL activity using an Escherichia coli protein expression system
ABL, a human tyrosine protein kinase, and its substrate are co-expressed in Escherichia coli. Tyrosine phosphorylation of the substrate in E. coli was detected using Phos-tag SDS-PAGE. The bacterial co-expression system was used as a field for the kinase reaction to evaluate the enzymatic activity o...
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Détails bibliographiques
| Publié dans: | BioTechniques. - 1993. - 70(2021), 4 vom: 01. Apr., Seite 209-217
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| Auteur principal: |
Kinoshita-Kikuta, Emiko
(Auteur) |
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| Autres auteurs: |
Yoshimoto, Momoka,
Yano, Marina,
Kinoshita, Eiji,
Koike, Tohru |
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| Format: | Article en ligne
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| Langue: | English |
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| Publié: |
2021
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| Accès à la collection: | BioTechniques
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| Sujets: | Journal Article
Research Support, Non-U.S. Gov't
ABL
Escherichia coli
abltide
co-expression
phos-tag
tyrosine protein kinase
Escherichia coli Proteins
Pyrimidines
plus...
Imatinib Mesylate
8A1O1M485B
Fusion Proteins, bcr-abl
EC 2.7.10.2 |
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