Co-suppression in Pyropia yezoensis (Rhodophyta) Reveals the Role of PyLHCI in Light Harvesting and Generation Switch
© 2020 Phycological Society of America.
| Veröffentlicht in: | Journal of phycology. - 1966. - 57(2021), 1 vom: 15. Feb., Seite 160-171 |
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| Weitere Verfasser: | , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2021
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| Zugriff auf das übergeordnete Werk: | Journal of phycology |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Pyropia yezoensis LHCI co-suppression generation switch life cycle |
| Zusammenfassung: | © 2020 Phycological Society of America. The red macroalga Pyropia yezoensis is an economically important seaweed widely cultured in Asian countries and is a model organism for molecular biological and commercial research. This species is unique in that it utilizes both phycobilisomes and transmembrane light-harvesting proteins as its antenna system. Here, one of the genes of P. yezoensis (PyLHCI) was selected for introduction into its genome to overexpress PyLHCI. However, the co-suppression phenomenon occurred. This is the first documentation of co-suppression in algae, in which it exhibits a different mechanism from that in higher plants. The transformant (T1) was demonstrated to have higher phycobilisomes and lower LHC binding pigments, resulting in a redder color, higher sensitivity to salt stress, smaller in size, and slower growth rate than the wildtype (WT). The photosynthetic performances of T1 and WT showed similar characteristics; however, P700 reduction was slower in T1. Most importantly, T1 could release a high percentage of carpospores in young blades to switch generation during its life cycle, which was rarely seen in WT. The co-suppression of PyLHCI revealed its key roles in light harvesting, stress resistance, and generation alternation (generation switch from gametophytes to sporophytes, and reproduction from asexual to sexual) |
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| Beschreibung: | Date Completed 20.04.2021 Date Revised 20.04.2021 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1529-8817 |
| DOI: | 10.1111/jpy.13073 |