Single-step colony assay with autoinduction of scFv expression for the screening of antibody libraries
We present a simple colony assay method for screening antibody libraries based on autoinduction of antibody fragment expression. This protocol eliminates the need for colony size monitoring and a separate induction step for single-chain Fv (scFv) antibody fragment expression. Here, scFvs are express...
| Publié dans: | BioTechniques. - 1991. - 66(2019), 4 vom: 20. Apr., Seite 194-197 |
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| Auteur principal: | |
| Autres auteurs: | |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2019
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article colony assay monoclonal antibody scFv screening Antibodies, Monoclonal Immunoglobulin G Peptide Library Single-Chain Antibodies |
| Résumé: | We present a simple colony assay method for screening antibody libraries based on autoinduction of antibody fragment expression. This protocol eliminates the need for colony size monitoring and a separate induction step for single-chain Fv (scFv) antibody fragment expression. Here, scFvs are expressed in an automatic and timely fashion during the assay, resulting in high yields of positive clones and substantial time savings. The method was used successfully to establish monoclonal scFvs with high affinity and specificity against human IgG |
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| Description: | Date Completed 31.01.2020 Date Revised 31.01.2020 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1940-9818 |
| DOI: | 10.2144/btn-2018-0195 |