MicroR408 regulates defense response upon wounding in sweet potato

MiRNAs play diverse roles in plant development and defense responses by binding to their mRNA targets based on sequence complementarity. Here, we investigated a wound-related miR408 and its target genes in sweet potato (Ipomoea batatas) by small RNA deep sequencing and transcriptome analysis. The ex...

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Détails bibliographiques
Publié dans:Journal of experimental botany. - 1985. - 70(2019), 2 vom: 07. Jan., Seite 469-483
Auteur principal: Kuo, Yun-Wei (Auteur)
Autres auteurs: Lin, Jeng-Shane, Li, Yu-Chi, Jhu, Min-Yao, King, Yu-Chi, Jeng, Shih-Tong
Format: Article en ligne
Langue:English
Publié: 2019
Accès à la collection:Journal of experimental botany
Sujets:Journal Article Research Support, Non-U.S. Gov't MicroRNAs
Description
Résumé:MiRNAs play diverse roles in plant development and defense responses by binding to their mRNA targets based on sequence complementarity. Here, we investigated a wound-related miR408 and its target genes in sweet potato (Ipomoea batatas) by small RNA deep sequencing and transcriptome analysis. The expression patterns of miR408 and the miR408 precursor were significantly repressed by wounding and jasmonate (JA). In contrast, expression of the putative target genes IbKCS (3-ketoacyl-CoA synthase 4), IbPCL (plantacyanin), and IbGAUT (galacturonosyltransferase 7-like) of miR408 was increased following wounding, whereas only IbKCS was increased after JA treatment. Target cleavage site mapping and Agrobacterium-mediated transient assay demonstrated that IbKCS, IbPCL, and IbGAUT were the targets of miR408. The expression of miR408 target genes was repressed in transgenic sweet potatoes overexpressing miR408. These data indicated a relationship between miR408 and its target genes. Notably, miR408-overexpressing plants showed a semi-dwarf phenotype and attenuated resistance to insect feeding, while transgenic plants overexpressing IbKCS exhibited more insect resistance than plants overexpressing only the empty vector. Collectively, sweet potato reduces the abundance of miR408 upon wounding to elevate the expression of IbKCS, IbPCL, and IbGAUT. The expression of IbKCS enhances the defense system against herbivore wounding
Description:Date Completed 11.02.2020
Date Revised 04.11.2023
published: Print
Citation Status MEDLINE
ISSN:1460-2431
DOI:10.1093/jxb/ery381