TEG-seq : an ion torrent-adapted NGS workflow for in cellulo mapping of CRISPR specificity
GUIDE-seq was developed to detect CRISPR/Cas9 off-target. However, as originally reported, it was associated with a high level of nonspecific amplification. In an attempt to improve it, we developed target-enriched GUIDE-seq (TEG-seq). The sensitivity level reached 0.1-10 reads-per-million dependin...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 65(2018), 5 vom: 25. Nov., Seite 259-267
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| 1. Verfasser: |
Tang, Pei-Zhong
(VerfasserIn) |
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| Weitere Verfasser: |
Ding, Bo,
Peng, Lansha,
Mozhayskiy, Vadim,
Potter, Jason,
Chesnut, Jonathan D |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2018
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
CRISPR/cas9
GUIDE-seq
TEG-seq
gene editing
off-target
RNA, Guide, CRISPR-Cas Systems
Ribonucleoproteins |
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