A PAGE screening approach for identifying CRISPR-Cas9-induced mutations in zebrafish

The introduction of CRISPR-Cas9 technology for targeted mutagenesis has revolutionized reverse genetics and made genome editing a realistic option in many model organisms. One of the difficulties with this technique is screening for mutations in large numbers of samples. Many screening approaches fo...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 64(2018), 6 vom: 06. Juni, Seite 275-278
1. Verfasser: VanLeuven, Ariel J (VerfasserIn)
Weitere Verfasser: Park, Sungdae, Menke, Douglas B, Lauderdale, James D
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2018
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, N.I.H., Extramural Danio rerio genotyping polymerase chain reaction (PCR) DNA 9007-49-2