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231225s2017 xx |||||o 00| ||eng c |
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|a 10.1107/S160057751701030X
|2 doi
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|a pubmed25n0917.xml
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|a (DE-627)NLM27534875X
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|a (NLM)28862633
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Kubo, Minoru
|e verfasserin
|4 aut
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|a Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA
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|c 2017
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Revised 03.06.2024
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a X-ray free-electron lasers (XFELs) have opened new opportunities for time-resolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TR-SFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented
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|a Journal Article
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|a XFEL
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|a pump and probe
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|a serial femtosecond crystallography
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|a time-resolved X-ray crystallography
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|a Nango, Eriko
|e verfasserin
|4 aut
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|a Tono, Kensuke
|e verfasserin
|4 aut
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|a Kimura, Tetsunari
|e verfasserin
|4 aut
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|a Owada, Shigeki
|e verfasserin
|4 aut
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|a Song, Changyong
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|4 aut
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|a Mafuné, Fumitaka
|e verfasserin
|4 aut
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|a Miyajima, Ken
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|4 aut
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|a Takeda, Yoshihiro
|e verfasserin
|4 aut
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|a Kohno, Jun Ya
|e verfasserin
|4 aut
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|a Miyauchi, Naoya
|e verfasserin
|4 aut
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|a Nakane, Takanori
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|4 aut
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|a Tanaka, Tomoyuki
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|4 aut
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|a Nomura, Takashi
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|4 aut
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|a Davidsson, Jan
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|4 aut
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|a Tanaka, Rie
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|4 aut
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|a Murata, Michio
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|4 aut
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|a Kameshima, Takashi
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|4 aut
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|a Hatsui, Takaki
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|4 aut
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|a Joti, Yasumasa
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|4 aut
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|a Neutze, Richard
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|4 aut
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|a Yabashi, Makina
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|a Iwata, So
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of synchrotron radiation
|d 1994
|g 24(2017), Pt 5 vom: 01. Sept., Seite 1086-1091
|w (DE-627)NLM09824129X
|x 1600-5775
|7 nnns
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| 773 |
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|g volume:24
|g year:2017
|g number:Pt 5
|g day:01
|g month:09
|g pages:1086-1091
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|u http://dx.doi.org/10.1107/S160057751701030X
|3 Volltext
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|d 24
|j 2017
|e Pt 5
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|c 09
|h 1086-1091
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