Characterization and fine-mapping of a novel premature leaf senescence mutant yellow leaf and dwarf 1 in rice

Copyright © 2016 Elsevier Masson SAS. All rights reserved.

Détails bibliographiques
Publié dans:Plant physiology and biochemistry : PPB. - 1991. - 111(2017) vom: 01. Feb., Seite 50-58
Auteur principal: Deng, Luchang (Auteur)
Autres auteurs: Qin, Peng, Liu, Zhi, Wang, Geling, Chen, Weilan, Tong, Jianhua, Xiao, Langtao, Tu, Bin, Sun, Yuantao, Yan, Wei, He, Hang, Tan, Jun, Chen, Xuewei, Wang, Yuping, Li, Shigui, Ma, Bingtian
Format: Article en ligne
Langue:English
Publié: 2017
Accès à la collection:Plant physiology and biochemistry : PPB
Sujets:Journal Article Chloroplast Leaf senescence MutMap ROS Reactive oxygen species SA SNP Salicylic acid Single nucleotide polymorphism plus... WT Wild type YLD Yellow leaf and dwarf Pigments, Biological Plant Growth Regulators Plant Proteins Salicylic Acid O414PZ4LPZ
Description
Résumé:Copyright © 2016 Elsevier Masson SAS. All rights reserved.
Leaves are the main organs in which photosynthates are produced. Leaf senescence facilitates the translocation of photosynthates and nutrients from source to sink, which is important for plant development and especially for crop yield. However, the molecular mechanism of leaf senescence is unknown. Here, we identified a mutant, yellow leaf and dwarf 1 (yld1), which exhibited decreased plant height and premature leaf senescence. Nitroblue tetrazolium and diamiobenzidine staining analyses revealed that the concentrations of reactive oxygen species were higher in yld1 leaves than in wild type leaves. The photosynthetic pigment contents were significantly decreased in yld1. The yld1 chloroplasts had collapsed and were filled with abnormal starch granules. Combining bulk segregant and MutMap gene mapping approaches, the mutation responsible for the yld1 phenotype was mapped to a 7.3 Mb centromeric region, and three non-synonymous single nucleotide polymorphisms located in three novel genes were identified in this region. The expression patterns of the three candidate genes indicated that LOC_Os06g29380 had the most potential for functional verification. Plant hormone measurements showed that salicylic acid was highly accumulated in yld1 leaves when compared with wild type leaves, and yld1 was more sensitive to salicylic acid than wild type. This work lays the foundation for understanding the molecular regulatory mechanism of leaf senescence, and may reveal new connections among the molecular pathways related to leaf senescence, starch metabolism and salicylic acid signaling
Description:Date Completed 01.05.2017
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2016.11.012