Two-color fluorescence in situ hybridization using chromogenic substrates in Xenopus

Xenopus embryo yolk proteins present a serious barrier to fluorescence microscopy. Previously, in situ assays of gene transcripts in whole embryos was limited to the use of chromogenic alkaline phosphatase substrates, which restricted researchers' ability to gauge coexpression of transcripts. H...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 61(2016), 5 vom: 01., Seite 263-268
1. Verfasser: Agricola, Zachary (VerfasserIn)
Weitere Verfasser: Cha, Sang-Wook
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, N.I.H., Extramural BCIP FISH NBT Vector Red Xenopus confocal fluorescent in situ hybridization two color mehr... Chromogenic Compounds Fluorescent Dyes Recombinant Proteins