Light-Patterned RNA Interference of 3D-Cultured Human Embryonic Stem Cells

Light-Patterned RNA Interference of 3D-Cultured Human Embryonic Stem Cells

© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Détails bibliographiques
Publié dans:Advanced materials (Deerfield Beach, Fla.). - 1998. - 28(2016), 48 vom: 16. Dez., Seite 10732-10737
Auteur principal: Huang, Xiao (Auteur)
Autres auteurs: Hu, Qirui, Lai, Yifan, Morales, Demosthenes P, Clegg, Dennis O, Reich, Norbert O
Format: Article en ligne
Langue:English
Publié: 2016
Accès à la collection:Advanced materials (Deerfield Beach, Fla.)
Sujets:Journal Article 3D cultures RNAi human embryonic stem cells near-infrared light spatial control RNA, Small Interfering Gold 7440-57-5
Description
Résumé:© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
A new method of spatially controlled gene regulation in 3D-cultured human embryonic stem cells is developed using hollow gold nanoshells (HGNs) and near-infrared (NIR) light. Targeted cell(s) are discriminated from neighboring cell(s) by focusing NIR light emitted from a two-photon microscope. Irradiation of cells that have internalized HGNs releases surface attached siRNAs and leads to concomitant gene downregulation
Description:Date Completed 20.09.2018
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-4095
DOI:10.1002/adma.201603318