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|a eng
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| 100 |
1 |
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|a Sola, Laura
|e verfasserin
|4 aut
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| 245 |
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|a Synthesis of Clickable Coating Polymers by Postpolymerization Modification
|b Applications in Microarray Technology
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|c 2016
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|a Text
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|a ƒaComputermedien
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|a Date Completed 06.06.2018
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|a Date Revised 06.06.2018
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a In this paper, we report on the postpolymerization modification (PPM) of a polymer to introduce new functionalities that enable click chemistry reactions for microarray applications. The parent polymer, named copoly(DMA-NAS-MAPS), is composed of N,N-dimethylacrylamide (DMA), a monomer that self-adsorbs onto different materials through weak interactions such as hydrogen bonding or van der Waals forces, 3-(trimethoxysilyl)propyl methacrylate (MAPS) that strengthens the stability of the coating through the formation of covalent bonds with siloxane groups on the surface to be coated, and N-acryloyloxysuccinimide (NAS), an active ester group, highly reactive toward nucleophiles, which enables bioprobe immobilization. This copolymer has been widely exploited to coat surfaces for microarray applications but exhibits some limitations because of the potential hydrolysis of the active ester (NHS ester). The degradation of the NHS ester hampers the use of this coating in some situations, for example, when probe immobilization cannot be accomplished through a microspotting situation, but in large volumes, for example, in microchannel derivatization or micro-/nanoparticle functionalization. To overcome the limitations of NHS esters, we have developed a family of polymers that originate from the common copolymer precursor, by reacting the active ester contained in the polymer chain with a bifunctional amine. In particular, the functional groups introduced in the polymer using PPM enable click chemistry reactions such as azide/alkyne or thiol/maleimide "click" reactions, with suitably modified biomolecules. The advantages of such reactions are quantitative yields, orthogonality of functional groups, and insensitivity of the reaction to pH. The new click functionalities, inserted with quantitative yields, improve the stability of the coating, enabling the attachment of biomolecules directly from a solution and avoiding the spotting of reduced volumes (pL) of probes. Finally, we have demonstrated the applicability of the click surfaces in a highly effective solid-phase PCR for the genotyping of the G12D KRAS mutation
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|a Journal Article
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|a Damin, Francesco
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gagni, Paola
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Consonni, Roberto
|e verfasserin
|4 aut
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| 700 |
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|a Chiari, Marcella
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 32(2016), 40 vom: 11. Okt., Seite 10284-10295
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|x 1520-5827
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|g year:2016
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|g month:10
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