Cytokinin metabolism in maize Novel evidence of cytokinin abundance, interconversions and formation of a new trans-zeatin metabolic product with a weak anticytokinin activity

Cytokinin metabolism in maize : Novel evidence of cytokinin abundance, interconversions and formation of a new trans-zeatin metabolic product with a weak anticytokinin activity

Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant science : an international journal of experimental plant biology. - 1985. - 247(2016) vom: 11. Juni, Seite 127-37
1. Verfasser: Hluska, Tomáš (VerfasserIn)
Weitere Verfasser: Dobrev, Petre I, Tarkowská, Dana, Frébortová, Jitka, Zalabák, David, Kopečný, David, Plíhal, Ondřej, Kokáš, Filip, Briozzo, Pierre, Zatloukal, Marek, Motyka, Václav, Galuszka, Petr
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Plant science : an international journal of experimental plant biology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Anticytokinin Cytokinin Kernels Metabolism Zea mays L. cis-zeatin trans-zeatin Cytokinins mehr... Plant Growth Regulators Plant Proteins Terpenes Zeatin 7I6OOJ9GR6 Oxidoreductases EC 1.- Glycosyltransferases EC 2.4.-
Beschreibung
Zusammenfassung:Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.
Cytokinins (CKs) are an important group of phytohormones. Their tightly regulated and balanced levels are essential for proper cell division and plant organ development. Here we report precise quantification of CK metabolites and other phytohormones in maize reproductive organs in the course of pollination and kernel maturation. A novel enzymatic activity dependent on NADP(+) converting trans-zeatin (tZ) to 6-(3-methylpyrrol-1-yl)purine (MPP) was detected. MPP shows weak anticytokinin properties and inhibition of CK dehydrogenases due to their ability to bind to an active site in the opposite orientation than substrates. Although the physiological significance of tZ side-chain cyclization is not anticipated as the MPP occurrence in maize tissue is very low, properties of the novel CK metabolite indicate its potential for utilization in plant in vitro tissue culture. Furthermore, feeding experiments with different isoprenoid CKs revealed distinct preferences in glycosylation of tZ and cis-zeatin (cZ). While tZ is preferentially glucosylated at the N9 position, cZ forms mainly O-glucosides. Since O-glucosides, in contrast to N9-glucosides, are resistant to irreversible cleavage catalyzed by CK dehydrogenases, the observed preference of maize CK glycosyltransferases to O-glycosylate zeatin in the cis-position might be a reason why cZ derivatives are over-accumulated in different maize tissues and organs
Beschreibung:Date Completed 14.12.2016
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2259
DOI:10.1016/j.plantsci.2016.03.014