IMPROVED METHODS FOR THE ISOLATION OF CYANOBACTERIAL DNA FROM ENVIRONMENTAL SAMPLES(1)
© 2009 Phycological Society of America.
| Publié dans: | Journal of phycology. - 1966. - 45(2009), 2 vom: 19. Apr., Seite 517-21 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2009
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| Accès à la collection: | Journal of phycology |
| Sujets: | Journal Article DNA PEG PVPP cyanobacteria fluorometry humics lake water microcystin sediment |
| Résumé: | © 2009 Phycological Society of America. DNA isolated from environmental samples often contains enzyme inhibitors disruptive to downstream molecular applications. Most of the existing methods of cyanobacterial DNA isolation do not effectively eliminate these inhibitors from sediment samples or cells collected from freshwater ecosystems. We describe improved methods based on the xanthogenate-SDS nucleic acid isolation (XS) method of Tillett and Neilan (2000). Our improved methods provided high-quality cyanobacterial DNA that could be amplified in PCR and digested with a restriction enzyme. Results were superior to several commercial kits. The DNA yield was also similar to that obtained via the standard XS method. These methods should provide valuable new tools for the expanded application of molecular genetics to limnological and oceanographic research |
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| Description: | Date Completed 04.04.2016 Date Revised 01.04.2016 published: Print GENBANK: FJ160469, FJ160479 Citation Status PubMed-not-MEDLINE |
| ISSN: | 1529-8817 |
| DOI: | 10.1111/j.1529-8817.2009.00651.x |