DIEL VARIATIONS OF CARBOHYDRATES AND NEUTRAL LIPIDS IN NITROGEN-SUFFICIENT AND NITROGEN-STARVED CYCLOSTAT CULTURES OF ISOCHRYSIS SP.(1)

© 2012 Phycological Society of America.

Détails bibliographiques
Publié dans:Journal of phycology. - 1966. - 48(2012), 4 vom: 24. Aug., Seite 966-75
Auteur principal: Lacour, Thomas (Auteur)
Autres auteurs: Sciandra, Antoine, Talec, Amélie, Mayzaud, Patrick, Bernard, Olivier
Format: Article en ligne
Langue:English
Publié: 2012
Accès à la collection:Journal of phycology
Sujets:Journal Article Isochrysis carbohydrate glycolipids light/dark cycle neutral lipid nitrogen starvation phospholipids
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245 1 0 |a DIEL VARIATIONS OF CARBOHYDRATES AND NEUTRAL LIPIDS IN NITROGEN-SUFFICIENT AND NITROGEN-STARVED CYCLOSTAT CULTURES OF ISOCHRYSIS SP.(1) 
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520 |a The goal of this study was to investigate the time response of two major carbon (C) reserves, respectively neutral lipids (NL) and total carbohydrate (TC), in the Haptophyte Isochrysis sp. growing in nitrogen (N)-sufficient or N-starved conditions and under light:dark (L:D) cycles. Experiments were carried out in a cyclostat culture system that allowed the following of the dynamics of the main cell compounds at both hourly and daily time scales. Under N-sufficient conditions, the L:D cycles cause the population to be synchronized, with most of the cells dividing at the beginning of the dark period. The C-specific growth rate was maximal around midday and negative during the dark period due to respiration processes. NL and TC both accumulated during the day and consumed during the night. We showed that NL and TC are highly dynamic compounds, as more than three quarters of NL and TC accumulated during the light period were consumed during the dark period. In contrast to NL, phospholipid and glycolipid to C ratios remained quite stable during the light/dark cycles. The major effect of N starvation on the NL and TC dynamics was to uncouple their diel variations from the L:D cycle, in two different ways depending on their respective role during short-term acclimation. Whereas the TC per cell ratio increased rapidly to reach a stable value in response to N starvation, NL per cell continued to oscillate, but with a pattern out of phase with the L:D cycle 
650 4 |a Journal Article 
650 4 |a Isochrysis 
650 4 |a carbohydrate 
650 4 |a glycolipids 
650 4 |a light/dark cycle 
650 4 |a neutral lipid 
650 4 |a nitrogen starvation 
650 4 |a phospholipids 
700 1 |a Sciandra, Antoine  |e verfasserin  |4 aut 
700 1 |a Talec, Amélie  |e verfasserin  |4 aut 
700 1 |a Mayzaud, Patrick  |e verfasserin  |4 aut 
700 1 |a Bernard, Olivier  |e verfasserin  |4 aut 
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