Using ApoE Nanolipoprotein Particles To Analyze SNARE-Induced Fusion Pores

Here we introduce ApoE-based nanolipoprotein particle (NLP)-a soluble, discoidal bilayer mimetic of ∼23 nm in diameter, as fusion partners to study the dynamics of fusion pores induced by SNARE proteins. Using in vitro lipid mixing and content release assays, we report that NLPs reconstituted with s...

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Publié dans:	Langmuir : the ACS journal of surfaces and colloids. - 1985. - 32(2016), 12 vom: 29. März, Seite 3015-23
Auteur principal:	Bello, Oscar D (Auteur)
Autres auteurs:	Auclair, Sarah M, Rothman, James E, Krishnakumar, Shyam S
Format:	Article en ligne
Langue:	English
Publié:	2016
Accès à la collection:	Langmuir : the ACS journal of surfaces and colloids
Sujets:	Journal Article Research Support, N.I.H., Extramural Apolipoproteins E Dextrans Fluorescent Dyes Liposomes Phosphatidylcholines Phosphatidylethanolamines Phosphatidylinositol 4,5-Diphosphate Phosphatidylinositols plus... Phosphatidylserines Synaptosomal-Associated Protein 25 Syntaxin 1 Vesicle-Associated Membrane Protein 2 Dithionite 14844-07-6 N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)phosphatidylethanolamine 64205-19-2 1,2-dioleoylphosphatidylserine 70614-14-1 Cholesterol 97C5T2UQ7J Calcium SY7Q814VUP 1-palmitoyl-2-oleoylphosphatidylcholine TE895536Y5 Dimyristoylphosphatidylcholine U86ZGC74V5 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine VM33LRU3A6