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231224s2016 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.5b04247
|2 doi
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|a pubmed25n0860.xml
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|a (DE-627)NLM258151102
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|a (NLM)26948099
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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|a Wang, Congzhou
|e verfasserin
|4 aut
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| 245 |
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|a Real-Time Observation of Antimicrobial Polycation Effects on Escherichia coli
|b Adapting the Carpet Model for Membrane Disruption to Quaternary Copolyoxetanes
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|c 2016
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 13.03.2017
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|a Date Revised 13.03.2017
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Real-time atomic force microscopy (AFM) was used for analyzing effects of the antimicrobial polycation copolyoxetane P[(C12)-(ME2Ox)-50/50], C12-50 on the membrane of a model bacterium, Escherichia coli (ATCC# 35218). AFM imaging showed cell membrane changes with increasing C12-50 concentration and time including nanopore formation and bulges associated with outer bacterial membrane disruption. A macroscale bactericidal concentration study for C12-50 showed a 4 log kill at 15 μg/mL with conditions paralleling imaging (1 h, 1x PBS, physiological pH, 25 °C). The dramatic changes from the control image to 1 h after introducing 15 μg/mL C12-50 are therefore reasonably attributed to cell death. At the highest concentration (60 μg/mL) further cell membrane disruption results in leakage of cytoplasm driven by detergent-like action. The sequence of processes for initial membrane disruption by the synthetic polycation C12-50 follows the carpet model posited for antimicrobial peptides (AMPs). However, the nanoscale details are distinctly different as C12-50 is a synthetic, water-soluble copolycation that is best modeled as a random coil. In a complementary AFM study, chemical force microscopy shows that incubating cells with C12-50 decreased the hydrophobicity across the entire cell surface at an early stage. This finding provides additional evidence indicating that C12-50 polycations initially bind with the cell membrane in a carpet-like fashion. Taken together, real time AFM imaging elucidates the mechanism of antimicrobial action for copolyoxetane C12-50 at the single cell level. In future work this approach will provide important insights into structure-property relationships and improved antimicrobial effectiveness for synthetic amphiphilic polycations
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Anti-Bacterial Agents
|2 NLM
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|a Polyurethanes
|2 NLM
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|a Surface-Active Agents
|2 NLM
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|a copolyoxetane C12-50
|2 NLM
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|a Polylysine
|2 NLM
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|a 25104-18-1
|2 NLM
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|a Zolotarskaya, Olga Y
|e verfasserin
|4 aut
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|a Nair, Sithara S
|e verfasserin
|4 aut
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|a Ehrhardt, Christopher J
|e verfasserin
|4 aut
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|a Ohman, Dennis E
|e verfasserin
|4 aut
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|a Wynne, Kenneth J
|e verfasserin
|4 aut
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|a Yadavalli, Vamsi K
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 32(2016), 12 vom: 29. März, Seite 2975-84
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnas
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| 773 |
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|g volume:32
|g year:2016
|g number:12
|g day:29
|g month:03
|g pages:2975-84
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|u http://dx.doi.org/10.1021/acs.langmuir.5b04247
|3 Volltext
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