Dehydrins from wheat x Thinopyrum ponticum amphiploid increase salinity and drought tolerance under their own inducible promoters without growth retardation

Copyright © 2015 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 99(2016) vom: 16. Feb., Seite 142-9
1. Verfasser: Qin, Yu-Xiang (VerfasserIn)
Weitere Verfasser: Qin, Fangyuan
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2016
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Abiotic stress Dehydrin Inducible promoter Wheat Sodium Chloride 451W47IQ8X Abscisic Acid 72S9A8J5GW
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245 1 0 |a Dehydrins from wheat x Thinopyrum ponticum amphiploid increase salinity and drought tolerance under their own inducible promoters without growth retardation 
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520 |a Copyright © 2015 Elsevier Masson SAS. All rights reserved. 
520 |a Dehydrins confer abiotic stress tolerance in seedlings, but few dehydrins have been studied by transgenic analysis under their own promoters in relation to abiotic stress tolerance. Also the inducible promoters for transgenic engineering are limited. In this study, we isolated from wheat three salt-induced YSK2 dehydrin genes and their promoters. The cDNA sequences were 711, 785, and 932 bp in length, encoding proteins containing 133, 166 and 231 amino acids, respectively, and were named TaDHN1, TaDHN2, and TaDHN3. TaDHN2 doesn't contain introns, while the other two genes each contain one. Semi-quantitative reverse transcription PCR analysis revealed all three dehydrin genes are substantially induced by ABA and NaCl, but only TaDHN2 is induced in seedlings by PEG and by cold (4 °C). Regulatory sequences upstream of the first translation codon (775, 1615 and 889 bp) of the three dehydrin genes were also cloned. Cis-element prediction indicated the presence of ABRE and other abiotic-stress-related elements. Histochemical analysis using GUS expression demonstrated that all three promoters were induced by ABA, cold or NaCl. Ectopic over-expression of TaDHN1 or TaDHN3 in Arabidopsis under their own inducible promoters enhanced NaCl- and drought-stress tolerance without growth retardation 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Abiotic stress 
650 4 |a Dehydrin 
650 4 |a Inducible promoter 
650 4 |a Wheat 
650 7 |a Sodium Chloride  |2 NLM 
650 7 |a 451W47IQ8X  |2 NLM 
650 7 |a Abscisic Acid  |2 NLM 
650 7 |a 72S9A8J5GW  |2 NLM 
700 1 |a Qin, Fangyuan  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant physiology and biochemistry : PPB  |d 1991  |g 99(2016) vom: 16. Feb., Seite 142-9  |w (DE-627)NLM098178261  |x 1873-2690  |7 nnns 
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