Heterotrimeric G proteins interact with defense-related receptor-like kinases in Arabidopsis
Copyright © 2015 Elsevier GmbH. All rights reserved.
| Publié dans: | Journal of plant physiology. - 1979. - 188(2015) vom: 01. Sept., Seite 44-8 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2015
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| Accès à la collection: | Journal of plant physiology |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Arabidopsis Heterotrimeric G proteins Plant defence Protein–protein interaction Receptor-like kinases (RLKs) Arabidopsis Proteins Protein Serine-Threonine Kinases EC 2.7.11.1 plus... |
| Résumé: | Copyright © 2015 Elsevier GmbH. All rights reserved. Heterotrimeric G proteins (G-proteins) are versatile signaling elements conserved in Eukaryotes. In animals G-proteins relay signals from 7-transmembrane spanning G protein-coupled receptors (GPCRs) to intracellular downstream effectors; however, the existence of GPCRs in plants is controversial. Contrastingly, a surplus of receptor-like kinases (RLKs) provides signal recognition at the plant cell surface. It is established that G proteins are involved in plant defense and suggested that they relay signals from defense-related RLKs. However, it is unclear how the signaling is conducted, as physical interaction between the RLKs and G proteins has not been demonstrated. Using yeast split-ubiquitin system and Bimolecular Fluorescence Complementation assays, we demonstrate physical interaction between the Gα, Gγ1 and Gγ2 subunits, and the defense-related RD-type receptor like kinases CERK1, BAK1 and BIR1. At the same time, no interaction was detected with the non-RD RLK FLS2. We hypothesize that G-proteins mediate signal transduction immediately downstream of the pathogenesis-related RLKs |
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| Description: | Date Completed 08.08.2016 Date Revised 03.12.2021 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1618-1328 |
| DOI: | 10.1016/j.jplph.2015.09.005 |