X-DNA origami-networked core-supported lipid stratum

DNA hydrogels are promising materials for various fields of research, such as in vitro protein production, drug carrier systems, and cell transplantation. For effective application and further utilization of DNA hydrogels, highly effective methods of nano- and microscale DNA hydrogel fabrication are...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1985. - 31(2015), 3 vom: 27. Jan., Seite 912-6
1. Verfasser: Shin, Seung Won (VerfasserIn)
Weitere Verfasser: Park, Kyung Soo, Jang, Min Su, Song, Woo Chul, Kim, Jin, Cho, Seung-Woo, Lee, Joo Young, Cho, Jeong Ho, Jung, Sunghwan, Um, Soong Ho
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2015
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Benzothiazoles DNA, Single-Stranded Diamines Fluorescent Dyes Hydrogels Organic Chemicals Phosphatidylcholines Phosphatidylethanolamines mehr... Phosphatidylglycerols Quinolines Viral Proteins Xanthenes SYBR Green I 163795-75-3 1,2-dipalmitoyl-3-phosphatidylethanolamine 3026-45-7 1-palmitoyl-2-oleoylglycero-3-phosphoglycerol 81490-05-3 Texas red 82354-19-6 Cholesterol 97C5T2UQ7J Ligases EC 6.- 1-palmitoyl-2-oleoylphosphatidylcholine TE895536Y5
Beschreibung
Zusammenfassung:DNA hydrogels are promising materials for various fields of research, such as in vitro protein production, drug carrier systems, and cell transplantation. For effective application and further utilization of DNA hydrogels, highly effective methods of nano- and microscale DNA hydrogel fabrication are needed. In this respect, the fundamental advantages of a core-shell structure can provide a simple remedy. An isolated reaction chamber and massive production platform can be provided by a core-shell structure, and lipids are one of the best shell precursor candidates because of their intrinsic biocompatibility and potential for easy modification. Here, we demonstrate a novel core-shell nanostructure made of gene-knitted X-shaped DNA (X-DNA) origami-networked gel core-supported lipid strata. It was simply organized by cross-linking DNA molecules via T4 enzymatic ligation and enclosing them in lipid strata. As a condensed core structure, the DNA gel shows Brownian behavior in a confined area. It has been speculated that they could, in the future, be utilized for in vitro protein synthesis, gene-integration transporters, and even new molecular bottom-up biological machineries
Beschreibung:Date Completed 30.09.2015
Date Revised 03.12.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1520-5827
DOI:10.1021/la503754e