Novel Method of Measuring Cantilever Deflection during an AFM Force Measurement
A combination of a reflection interference contrast microscope (RICM) and the atomic force microscope (AFM) was used to monitor the cantilever-surface separation distance during force measurements using the streptavidin-biotin recognition pairs. The RICM showed that the cantilever loses contact with...
| Publié dans: | Langmuir : the ACS journal of surfaces and colloids. - 1985. - 12(1996), 22 vom: 30. Okt., Seite 5244-5246 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
1996
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| Accès à la collection: | Langmuir : the ACS journal of surfaces and colloids |
| Sujets: | Journal Article |
| Résumé: | A combination of a reflection interference contrast microscope (RICM) and the atomic force microscope (AFM) was used to monitor the cantilever-surface separation distance during force measurements using the streptavidin-biotin recognition pairs. The RICM showed that the cantilever loses contact with the surface before the final rupture of the adhesive bonds is measured by the AFM detection system. This finding suggests that the immobilization of biotin by physisorbed albumin and subsequent binding of streptavidin might have created a cross-linked protein network whose cohesion is tested by the AFM cantilever with the immobilized biotin ligands |
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| Description: | Date Revised 29.05.2025 published: Print Citation Status PubMed-not-MEDLINE |
| ISSN: | 1520-5827 |