Reproducibility of nuclear morphometry parameters from cytologic smears of canine cutaneous mast cell tumors--intra- and interobserver variability

© 2014 American Society for Veterinary Clinical Pathology and European Society for Veterinary Clinical Pathology.

Bibliographische Detailangaben
Veröffentlicht in:Veterinary clinical pathology. - 1975. - 43(2014), 3 vom: 25. Sept., Seite 469-72
1. Verfasser: Barbosa, Daniella de F L (VerfasserIn)
Weitere Verfasser: Paraventi, Mariana D, Strefezzi, Ricardo de F
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2014
Zugriff auf das übergeordnete Werk:Veterinary clinical pathology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Grading mastocytoma observer experience prognosis
Beschreibung
Zusammenfassung:© 2014 American Society for Veterinary Clinical Pathology and European Society for Veterinary Clinical Pathology.
BACKGROUND: Qualitative and/or semiquantitative histopathologic grading has been the method of choice to predict the biologic behavior of mast cell tumors (MCT). Recently, computer-assisted nuclear morphometric analysis of histologic or cytologic specimens has been shown to correlate with histopathologic grading of MCT. However, it is still not clear what the reproducibility and variability of these computer-assisted measurements are
OBJECTIVES: The objective of this study was to estimate the intra- and interobserver variability in computer-assisted morphometric analysis of nuclear parameters in fine-needle cytologic smears of canine cutaneous MCT
MATERIALS AND METHODS: Measurements were performed by 2 observers with basic training on the recognition of neoplastic cells and operating the image analysis software. The analyses were performed twice at 6 months apart
RESULTS: No significant effects were detected for observer, time, and the interaction observer-time
CONCLUSIONS: Nuclear morphometric analysis of mast cell tumors using cytologic smears is a reproducible method for grading of MCT
Beschreibung:Date Completed 12.06.2015
Date Revised 20.10.2016
published: Print-Electronic
CommentIn: Vet Clin Pathol. 2014 Sep;43(3):305-9. doi: 10.1111/vcp.12191. - PMID 25154306
Citation Status MEDLINE
ISSN:1939-165X
DOI:10.1111/vcp.12167