HMA1 and PAA1, two chloroplast-envelope PIB-ATPases, play distinct roles in chloroplast copper homeostasis

Copper is an essential micronutrient but it is also potentially toxic as copper ions can catalyse the production of free radicals, which result in various types of cell damage. Therefore, copper homeostasis in plant and animal cells must be tightly controlled. In the chloroplast, copper import is me...

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Veröffentlicht in:	Journal of experimental botany. - 1985. - 65(2014), 6 vom: 01. Apr., Seite 1529-40
1. Verfasser:	Boutigny, Sylvain (VerfasserIn)
Weitere Verfasser:	Sautron, Emeline, Finazzi, Giovanni, Rivasseau, Corinne, Frelet-Barrand, Annie, Pilon, Marinus, Rolland, Norbert, Seigneurin-Berny, Daphné
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2014
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Arabidopsis PIB ATPase. chloroplast copper envelope transporter metal homeostasis Arabidopsis Proteins mehr... Copper 789U1901C5 Adenosine Triphosphatases EC 3.6.1.- HMA1 protein, Arabidopsis Chloroplast Proton-Translocating ATPases EC 3.6.3.- PAA1 protein, Arabidopsis

Beschreibung
Zusammenfassung:	Copper is an essential micronutrient but it is also potentially toxic as copper ions can catalyse the production of free radicals, which result in various types of cell damage. Therefore, copper homeostasis in plant and animal cells must be tightly controlled. In the chloroplast, copper import is mediated by a chloroplast-envelope PIB-type ATPase, HMA6/PAA1. Copper may also be imported by HMA1, another chloroplast-envelope PIB-ATPase. To get more insights into the specific functional roles of HMA1 and PAA1 in copper homeostasis, this study analysed the phenotypes of plants affected in the expression of both HMA1 and PAA1 ATPases, as well as of plants overexpressing HMA1 in a paa1 mutant background. The results presented here provide new evidence associating HMA1 with copper homeostasis in the chloroplast. These data suggest that HMA1 and PAA1 behave as distinct pathways for copper import and targeting to the chloroplast. Finally, this work also provides evidence for an alternative route for copper import into the chloroplast mediated by an as-yet unidentified transporter that is neither HMA1 nor PAA1
Beschreibung:	Date Completed 17.11.2014 Date Revised 19.11.2015 published: Print-Electronic Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/eru020