Fast mitochondrial DNA isolation from mammalian cells for next-generation sequencing
Standard methods for mitochondrial DNA (mtDNA) extraction do not provide the level of enrichment for mtDNA sufficient for direct sequencing and must be followed by long-range-PCR amplification, which can bias the sequencing results. Here, we describe a fast, cost-effective, and reliable method for p...
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Détails bibliographiques
| Publié dans: | BioTechniques. - 1991. - 55(2013), 3 vom: 18. Sept., Seite 133-6
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| Auteur principal: |
Quispe-Tintaya, Wilber
(Auteur) |
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| Autres auteurs: |
White, Ryan R,
Popov, Vasily N,
Vijg, Jan,
Maslov, Alexander Y |
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| Format: | Article en ligne
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| Langue: | English |
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| Publié: |
2013
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| Accès à la collection: | BioTechniques
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| Sujets: | Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
mitochondrial DNA
next-generation sequencing
DNA, Mitochondrial |
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