Fast mitochondrial DNA isolation from mammalian cells for next-generation sequencing
Standard methods for mitochondrial DNA (mtDNA) extraction do not provide the level of enrichment for mtDNA sufficient for direct sequencing and must be followed by long-range-PCR amplification, which can bias the sequencing results. Here, we describe a fast, cost-effective, and reliable method for p...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 55(2013), 3 vom: 18. Sept., Seite 133-6
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| 1. Verfasser: |
Quispe-Tintaya, Wilber
(VerfasserIn) |
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| Weitere Verfasser: |
White, Ryan R,
Popov, Vasily N,
Vijg, Jan,
Maslov, Alexander Y |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2013
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
mitochondrial DNA
next-generation sequencing
DNA, Mitochondrial |
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