Adjuvanted HLA-supertype restricted subdominant peptides induce new T-cell immunity during untreated HIV-1-infection

Adjuvanted HLA-supertype restricted subdominant peptides induce new T-cell immunity during untreated HIV-1-infection

Copyright © 2012 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 146(2013), 2 vom: 15. Feb., Seite 120-30
1. Verfasser: Karlsson, Ingrid (VerfasserIn)
Weitere Verfasser: Brandt, Lea, Vinner, Lasse, Kromann, Ingrid, Andreasen, Lars Vibe, Andersen, Peter, Gerstoft, Jan, Kronborg, Gitte, Fomsgaard, Anders
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Clinical Trial, Phase I Journal Article Randomized Controlled Trial Research Support, Non-U.S. Gov't Adjuvants, Immunologic Epitopes, T-Lymphocyte HLA-A Antigens HLA-B Antigens HLA-C Antigens Immunodominant Epitopes Peptides
Beschreibung
Zusammenfassung:Copyright © 2012 Elsevier Inc. All rights reserved.
We investigated the potential of inducing additional T-cell immunity during chronic HIV-1 infection directed to subdominant HIV-1 epitopes from common HLA-supertypes. Ten treatment-naïve HIV-1-infected individuals were immunized with peptides in the adjuvant CAF01. One individual received placebo. T-cell immunogenicity was examined longitudinally by a flow cytometry (CD107a, IFNγ, TNFα, IL-2 and/or MIP1β expression) as well as IFNγ ELISPOT. Safety was evaluated by clinical follow up combined with monitoring of biochemistry, hematology, CD4 T-cell counts and viral load. New CD4 and CD8 T-cell responses specific for one or more vaccine epitopes were induced in 10/10 vaccinees. The responses were dominated by CD107a and MIP1β expression. There were no significant changes in HIV-1 viral load or CD4 T-cell counts. Our study demonstrates that the peptide/CAF01 vaccine is safe and that it is possible to generate new HIV-1 T-cell responses to defined epitopes in treatment-naïve HIV-1-infected individuals
Beschreibung:Date Completed 01.04.2013
Date Revised 04.02.2013
published: Print-Electronic
ClinicalTrials.gov: NCT01009762
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2012.12.005