|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM224007572 |
| 003 |
DE-627 |
| 005 |
20250214202908.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2013 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1002/jcc.23213
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n0746.xml
|
| 035 |
|
|
|a (DE-627)NLM224007572
|
| 035 |
|
|
|a (NLM)23299435
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Choi, Yoonjoo
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Structure-based redesign of proteins for minimal T-cell epitope content
|
| 264 |
|
1 |
|c 2013
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 02.07.2014
|
| 500 |
|
|
|a Date Revised 23.03.2024
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Copyright © 2013 Wiley Periodicals, Inc.
|
| 520 |
|
|
|a The protein universe displays a wealth of therapeutically relevant activities, but T-cell driven immune responses to non-"self" biological agents present a major impediment to harnessing the full diversity of these molecular functions. Mutagenic T-cell epitope deletion seeks to mitigate the immune response, but can typically address only a small number of epitopes. Here, we pursue a "bottom-up" approach that redesigns an entire protein to remain native-like but contain few if any immunogenic epitopes. We do so by extending the Rosetta flexible-backbone protein design software with an epitope scoring mechanism and appropriate constraints. The method is benchmarked with a diverse panel of proteins and applied to three targets of therapeutic interest. We show that the deimmunized designs indeed have minimal predicted epitope content and are native-like in terms of various quality measures, and moreover that they display levels of native sequence recovery comparable to those of non-deimmunized designs
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, N.I.H., Extramural
|
| 650 |
|
7 |
|a Epitopes, T-Lymphocyte
|2 NLM
|
| 650 |
|
7 |
|a Proteins
|2 NLM
|
| 700 |
1 |
|
|a Griswold, Karl E
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Bailey-Kellogg, Chris
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of computational chemistry
|d 1984
|g 34(2013), 10 vom: 05. Apr., Seite 879-91
|w (DE-627)NLM098138448
|x 1096-987X
|7 nnns
|
| 773 |
1 |
8 |
|g volume:34
|g year:2013
|g number:10
|g day:05
|g month:04
|g pages:879-91
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1002/jcc.23213
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 34
|j 2013
|e 10
|b 05
|c 04
|h 879-91
|