Molecular and cytogenetic identification of new wheat-Dasypyrum breviaristatum additions conferring resistance to stem rust and powdery mildew

Two cytologically stable wheat-Dasypyrum breviarisatatum addition lines, Y93-1-6-6 and Y93-1-A6-4, were identified by integrated molecular and cytogenetic techniques. C-banding and genomic in situ hybridization (GISH) showed that Y93-1-6-6 and Y93-1-A6-4 were different wheat-D. breviaristatum additi...

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Publié dans:Breeding science. - 1998. - 61(2011), 4 vom: 01. Dez., Seite 366-72
Auteur principal: Liu, Cheng (Auteur)
Autres auteurs: Li, Guangrong, Yan, Hongfei, Zhou, Jianping, Hu, Lijun, Lei, Mengping, Ran, Ling, Yang, Zujun
Format: Article en ligne
Langue:English
Publié: 2011
Accès à la collection:Breeding science
Sujets:Journal Article Blumeria graminis tritici D. breviaristatum GISH Puccinia graminis tritici addition line
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520 |a Two cytologically stable wheat-Dasypyrum breviarisatatum addition lines, Y93-1-6-6 and Y93-1-A6-4, were identified by integrated molecular and cytogenetic techniques. C-banding and genomic in situ hybridization (GISH) showed that Y93-1-6-6 and Y93-1-A6-4 were different wheat-D. breviaristatum additions. A total of 51 markers (primer/enzyme combinations), including 6 PCR-based Landmark Unique Gene (PLUG) markers and 45 Sequence-Tagged-Site (STS) markers, were selected from 3,774 primer/enzyme combinations to further characterize these two additions. Marker haploytpes suggested that both D. breviaristatum chromosomes in Y93-1-6-6 and Y93-1-A6-4 were rearranged. Stem rust resistance screening indicated that both additions were highly resistant to race RKQQC, whereas only Y93-1-6-6 was resistant to race TTKSK (Ug99). Powdery mildew resistance screening showed that only Y93-1-6-6 was resistant. Pedigree analysis suggested that the stem rust and powdery mildew resistance of Y93-1-6-6 was derived from D. breviaristatum, indicating that the D. breviaristatum chromosomes in Y93-1-6-6 possess a new powdery mildew resistance gene(s), and new stem rust resistance gene(s). These two additions could be used as stem rust or powdery mildew resistance sources in wheat breeding programs 
650 4 |a Journal Article 
650 4 |a Blumeria graminis tritici 
650 4 |a D. breviaristatum 
650 4 |a GISH 
650 4 |a Puccinia graminis tritici 
650 4 |a addition line 
700 1 |a Li, Guangrong  |e verfasserin  |4 aut 
700 1 |a Yan, Hongfei  |e verfasserin  |4 aut 
700 1 |a Zhou, Jianping  |e verfasserin  |4 aut 
700 1 |a Hu, Lijun  |e verfasserin  |4 aut 
700 1 |a Lei, Mengping  |e verfasserin  |4 aut 
700 1 |a Ran, Ling  |e verfasserin  |4 aut 
700 1 |a Yang, Zujun  |e verfasserin  |4 aut 
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773 1 8 |g volume:61  |g year:2011  |g number:4  |g day:01  |g month:12  |g pages:366-72 
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