Identification of novel drought-related mRNAs in common bean roots by differential display RT-PCR
Copyright © 2006 Elsevier Ireland Ltd. All rights reserved.
| Publié dans: | Plant science : an international journal of experimental plant biology. - 1985. - 171(2006), 3 vom: 01. Sept., Seite 300-7 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2006
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| Accès à la collection: | Plant science : an international journal of experimental plant biology |
| Sujets: | Journal Article |
| Résumé: | Copyright © 2006 Elsevier Ireland Ltd. All rights reserved. Drought is a major constraint for the production of common bean (Phaseolus vulgaris L.). To identify molecular responses to water deficit, we performed a differential display RT-PCR (DDRT) analysis using roots of bean plants grown aeroponically and submitted to dehydration. This allowed us to visualise 1200 DDRT bands, 8.7% of which showed a clear regulation by dehydration, and to clone 42 cDNAs, called PvD1 to PvD42. Among them, 20 early-dehydration-responsive cDNAs were selected by reverse northern that were induced or repressed before detectable water status changes and induction of ABA-regulated genes. Northern analysis for 16 PvD clones confirmed these early regulations and allowed us to identify four late dehydration-responsive genes. Their putative involvement in signalling, protein turn-over and translocation, chaperones as well as root growth modulations in response to water stress is discussed |
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| Description: | Date Completed 02.10.2012 Date Revised 09.01.2024 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
| ISSN: | 1873-2259 |
| DOI: | 10.1016/j.plantsci.2006.03.008 |