Real-time surface-enhanced Raman spectroscopy monitoring of surface pH during electrochemical melting of double-stranded DNA

Bibliographische Detailangaben
Veröffentlicht in:	Langmuir : the ACS journal of surfaces and colloids. - 1985. - 28(2012), 12 vom: 27. März, Seite 5464-70
1. Verfasser:	Johnson, Robert P (VerfasserIn)
Weitere Verfasser:	Richardson, James A, Brown, Tom, Bartlett, Philip N
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2012
Zugriff auf das übergeordnete Werk:	Langmuir : the ACS journal of surfaces and colloids
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't DNA 9007-49-2

Beschreibung
Zusammenfassung:	© 2012 American Chemical Society The application of a negative potential ramp at a double-stranded DNA (dsDNA) functionalized electrode surface results in the gradual denaturation of the DNA in a process known as electrochemical melting. The underlying physical chemistry behind electrochemically driven DNA denaturation is not well understood, and one possible mechanism is a change in local pH at the electrode surface. We demonstrate that by coimmobilization of p-mercaptobenozic acid at a dsDNA-functionalized electrode surface, it is possible to monitor both DNA denaturation and the local pH simultaneously using surface-enhanced Raman spectroscopy. We find that the local pH at the electrode surface does not change as the applied potential is scanned negative and the dsDNA denatures. We therefore conclude that in these experiments electrochemical melting is not caused by electrochemically driven local pH changes
Beschreibung:	Date Completed 24.07.2012 Date Revised 27.03.2012 published: Print-Electronic Citation Status MEDLINE
ISSN:	1520-5827
DOI:	10.1021/la204794g