Global analysis of RNA oxidation in Saccharomyces cerevisiae
Oxidative RNA damage has been linked to loss of RNA function and to the development of many human age-related diseases. Consequently, a need exists for methods to identify and quantify the extent of RNA oxidation on a genome-wide basis. We developed such a method by combining affinity selection of m...
| Publié dans: | BioTechniques. - 1991. - 52(2012), 2 vom: 06. Feb., Seite 109-11 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2012
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| Accès à la collection: | BioTechniques |
| Sujets: | Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't RNA, Fungal Saccharomyces cerevisiae Proteins |
| Résumé: | Oxidative RNA damage has been linked to loss of RNA function and to the development of many human age-related diseases. Consequently, a need exists for methods to identify and quantify the extent of RNA oxidation on a genome-wide basis. We developed such a method by combining affinity selection of mRNA containing 8-hydroxyguanine with high throughput DNA sequencing. We demonstrate that this assay is suitable for detecting differences in the extent of oxidation between RNA transcripts. We applied this method to the yeast Saccharomyces cerevisiae grown under physiological conditions and in response to hydrogen peroxide, and detected significantly oxidized RNA transcripts |
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| Description: | Date Completed 01.10.2012 Date Revised 29.01.2022 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |
| DOI: | 10.2144/000113801 |