A strategy for purifying glutathione S-transferase in the presence of sodium dodecyl sulfate
Glutathione S-transferase (GST) is widely used to prepare and purify GSTtagged fusion proteins. Although GST improves protein solubility, detergents must often be used to achieve protein solubilization from bacterial lysates. However, purification of GST by affinity chromatography cannot be achieved...
| Publié dans: | BioTechniques. - 1991. - 51(2011), 3 vom: 01. Sept., Seite 193-4 |
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| Auteur principal: | |
| Autres auteurs: | , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2011
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Glycols Recombinant Fusion Proteins Sodium Dodecyl Sulfate 368GB5141J Glutathione Transferase EC 2.5.1.18 hexylene glycol KEH0A3F75J |
| Accès en ligne |
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