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231224s2011 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2011.07.004
|2 doi
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|a pubmed25n0703.xml
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|a (DE-627)NLM210888369
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|a (NLM)21856037
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Zhang, Yongqiang
|e verfasserin
|4 aut
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|a Gibberellins negatively regulate light-induced nitrate reductase activity in Arabidopsis seedlings
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|c 2011
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 09.04.2012
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|a Date Revised 30.09.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a Copyright © 2011 Elsevier GmbH. All rights reserved.
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|a In the present study, the role of phytohormone gibberellins (GAs) on regulating the nitrate reductase (NR) activity was tested in Arabidopsis seedlings. The NR activity in light-grown Col-0 seedlings was reduced by exogenous GA₃ (an active form of GAs), but enhanced by exogenous paclobutrazol (PAC, a gibberellin biosynthesis inhibitor), suggesting that GAs negatively regulate the NR activity in light-grown seedlings. Light is known to influence the NR activity through both photosynthesis and phytochromes. When etiolated seedlings were transferred to white or red light, both exogenously applied GA₃ and PAC were found to function on the NR activity only in the presence of sucrose, implying that GAs are not involved in light signaling-induced but negatively regulate photoproducts-induced NR activity. NR is regulated by light mainly at two levels: transcript level and post-translational level. Our reverse transcription (RT)-PCR assays showed that GAs did not affect the transcript levels of NIA1 and NIA2, two genes that encode NR proteins. But the divalent cations (especially Mg²⁺) were required for GAs negative regulation of NR activity, in view of the importance of divalent cations during the process of post-translational regulation of NR activity, which indicates that GAs very likely regulate the NR activity at the post-translational level. In the following dark-light shift analyses, GAs were found to accelerate dark-induced decrease, but retard light-induced increase of the NR activity. Furthermore, it was observed that application of G₃ or PAC could impair diurnal variation of the NR activity. These results collectively indicate that GAs play a negative role during light regulation of NR activity in nature
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Gibberellins
|2 NLM
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|a Triazoles
|2 NLM
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|a paclobutrazol
|2 NLM
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|a 6PLV42R3ZA
|2 NLM
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|a Nitrate Reductases
|2 NLM
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|a EC 1.7.-
|2 NLM
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|a Nitrate Reductase
|2 NLM
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| 650 |
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|a EC 1.7.99.4
|2 NLM
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| 700 |
1 |
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|a Liu, Zhongjuan
|e verfasserin
|4 aut
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| 700 |
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|a Liu, Rongzhi
|e verfasserin
|4 aut
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| 700 |
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|a Wang, Liguang
|e verfasserin
|4 aut
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| 700 |
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|a Bi, Yurong
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 168(2011), 18 vom: 15. Dez., Seite 2161-8
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnas
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| 773 |
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|g volume:168
|g year:2011
|g number:18
|g day:15
|g month:12
|g pages:2161-8
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|u http://dx.doi.org/10.1016/j.jplph.2011.07.004
|3 Volltext
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