Interface of covalently bonded phospholipids with a phosphorylcholine head characterization, protein nonadsorption, and further functionalization

Interface of covalently bonded phospholipids with a phosphorylcholine head : characterization, protein nonadsorption, and further functionalization

© 2011 American Chemical Society

Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1985. - 27(2011), 18 vom: 20. Sept., Seite 11536-44
1. Verfasser: Ferez, Lynda (VerfasserIn)
Weitere Verfasser: Thami, Thierry, Akpalo, Edefia, Flaud, Valérie, Tauk, Lara, Janot, Jean-Marc, Déjardin, Philippe
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2011
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Phospholipids Proteins Siloxanes poly(methylhydrosiloxane) Water 059QF0KO0R Phosphorylcholine 107-73-3
Beschreibung
Zusammenfassung:© 2011 American Chemical Society
Surface anchored poly(methylhydrosiloxane) (PMHS) thin films on oxidized silicon wafers or glass substrates were functionalized via the SiH hydrosilylation reaction with the internal double bonds of 1,2-dilinoleoyl-sn-glycero-3-phosphorylcholine (18:2 Cis). The surface was characterized by X-ray photoelectron spectroscopy, contact angle measurements, atomic force microscopy, and scanning electron microscopy. These studies showed that the PMHS top layer could be efficiently modified resulting in an interfacial high density of phospholipids. Grafted phospholipids made the initially hydrophobic surface (θ = 106°) very hydrophilic and repellent toward avidin, bovine serum albumin, bovine fibrinogen, lysozyme, and α-chymotrypsin adsorption in phosphate saline buffer pH 7.4. The surface may constitute a new background-stable support with increased biocompatibility. Further possibilities of functionalization on the surface remain available owing to the formation of interfacial SiOH groups by Karstedt-catalyzed side reactions of SiH groups with water. The presence of interfacial SiOH groups was shown by zeta potential measurements. The reactivity and surface density of SiOH groups were checked by fluorescence after reaction of a monoethoxy silane coupling agent bearing Alexa as fluorescent probe
Beschreibung:Date Completed 13.01.2012
Date Revised 02.02.2018
published: Print-Electronic
Citation Status MEDLINE
ISSN:1520-5827
DOI:10.1021/la202793k