The Arabidopsis Ca(2+) -dependent protein kinase CPK12 negatively regulates abscisic acid signaling in seed germination and post-germination growth

The Arabidopsis Ca(2+) -dependent protein kinase CPK12 negatively regulates abscisic acid signaling in seed germination and post-germination growth

© 2011 The Authors. New Phytologist © 2011 New Phytologist Trust.

Détails bibliographiques
Publié dans:The New phytologist. - 1990. - 192(2011), 1 vom: 01. Okt., Seite 61-73
Auteur principal: Zhao, Rui (Auteur)
Autres auteurs: Sun, Hai-Li, Mei, Chao, Wang, Xiao-Jing, Yan, Lu, Liu, Rui, Zhang, Xiao-Feng, Wang, Xiao-Fang, Zhang, Da-Peng
Format: Article en ligne
Langue:English
Publié: 2011
Accès à la collection:The New phytologist
Sujets:Journal Article Research Support, Non-U.S. Gov't Arabidopsis Proteins RNA, Messenger Transcription Factors Abscisic Acid 72S9A8J5GW Protein Kinases EC 2.7.- calcium-dependent protein kinase plus... EC 2.7.1.- CPK12 protein, Arabidopsis EC 2.7.11.17 Calcium-Calmodulin-Dependent Protein Kinases ABI2 protein, Arabidopsis EC 3.1.3.- Phosphoprotein Phosphatases EC 3.1.3.16
Description
Résumé:© 2011 The Authors. New Phytologist © 2011 New Phytologist Trust.
• Ca(2+) -dependent protein kinase (CDPK) is believed to be involved in abscisic acid (ABA) signaling, and several members of the Arabidopsis CDPK superfamily have been identified as positive ABA signaling regulators, but it remains unknown if CDPK negatively regulates ABA signaling. • Here, we investigated the function of an Arabidopsis (Arabidopsis thaliana) CDPK, CPK12, in ABA signaling pathway. • We generated Arabidopsis CPK12-RNAi lines, and observed that downregulation of CPK12 resulted in ABA hypersensitivity in seed germination and post-germination growth, and altered expression of a set of ABA-responsive genes. Expression assay showed that CPK12 was ubiquitously expressed and localized to both cytosol and nucleus. Biochemical assays showed that CPK12 interacted with, phosphorylated and stimulated a type 2C protein phosphatase ABI2, and phosphorylated two ABA-responsive transcription factors (ABF1 and ABF4) in vitro. • Our findings show that the Arabidopsis CPK12 is a negative ABA-signaling regulator in seed germination and post-germination growth, suggesting that different members of the CDPK family may constitute a regulation loop by functioning positively and negatively in ABA signal transduction
Description:Date Completed 30.12.2011
Date Revised 09.01.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/j.1469-8137.2011.03793.x