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231224s2011 xx |||||o 00| ||eng c |
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|a 10.1021/la2004694
|2 doi
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|a pubmed24n0691.xml
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|e rakwb
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|a eng
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1 |
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|a Lai, Siok Lian
|e verfasserin
|4 aut
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|a Enhancing the fluorescence intensity of DNA microarrays by using cationic surfactants
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 11.08.2011
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|a Date Revised 01.12.2018
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a DNA microarrays have been used as powerful tools in genomics studies and single nucleotide polymorphisms analysis. However, the fluorescence detection used in most conventional DNA microarrays is still limited by its sensitivity. The aim of this study is to use a cationic surfactant, cetyl trimethylammonium bromide (CTAB), to enhance the fluorescence intensity of 6-carboxy-fluorescene (FAM)-labeled DNA probes immobilized on a DNA microarray. We show that in the presence of CTAB the immobilized FAM-labeled DNA probes is 11-fold brighter than that without exposure to CTAB. Similarly, when we hybridize FAM-labeled DNA targets to a DNA microarray and treat the surface with CTAB solution, the fluorescence intensity shows a 26-fold increase for perfect-match DNA targets. More importantly, the contrast between perfect-match and 1-mismatch DNA is also increased from 1.3-fold to 15-fold. This method offers a simple and efficient technique to enhance the detection limit of DNA microarrays
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Carbocyanines
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|a Cetrimonium Compounds
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|a DNA Probes
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|a Fluoresceins
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|a Solutions
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|a Surface-Active Agents
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|a cyanine dye 3
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|a 6-carboxyfluorescein
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|a Chen, Chih-Hsin
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|a Yang, Kun-Lin
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 27(2011), 9 vom: 03. Mai, Seite 5659-64
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:27
|g year:2011
|g number:9
|g day:03
|g month:05
|g pages:5659-64
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|u http://dx.doi.org/10.1021/la2004694
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