Liquid scintillation spectrometry of tritium in studying lysozyme behavior in aqueous/organic liquid systems. The influence of the organic phase

Liquid scintillation spectrometry of tritium in the application of the scintillation phase method was used for studying the adsorption of lysozyme at the liquid/liquid interface and its distribution in the bulk of the system. The goal of this research was to reveal the influence of the nature of the...

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Détails bibliographiques
Publié dans:Langmuir : the ACS journal of surfaces and colloids. - 1985. - 27(2011), 6 vom: 15. März, Seite 2188-94
Auteur principal: Chernysheva, Maria G (Auteur)
Autres auteurs: Badun, Gennadii A
Format: Article en ligne
Langue:English
Publié: 2011
Accès à la collection:Langmuir : the ACS journal of surfaces and colloids
Sujets:Journal Article Research Support, Non-U.S. Gov't Octanes Octanols Xylenes Water 059QF0KO0R Tritium 10028-17-8 4-xylene plus... 6WAC1O477V Muramidase EC 3.2.1.17 octane X1RV0B2FJV
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Résumé:Liquid scintillation spectrometry of tritium in the application of the scintillation phase method was used for studying the adsorption of lysozyme at the liquid/liquid interface and its distribution in the bulk of the system. The goal of this research was to reveal the influence of the nature of the organic phase on the distribution and adsorption ability of the protein when it is placed in a system containing two immiscible liquids. Based on the radiochemical assay distribution coefficients and adsorption isotherms obtained for aqueous/octane, aqueous/p-xylene and aqueous/octanol systems, it was concluded that the interaction of the protein with the interface plays a dominant role in protein behavior in aqueous/organic liquid systems
Description:Date Completed 21.10.2014
Date Revised 04.02.2014
published: Print-Electronic
Citation Status MEDLINE
ISSN:1520-5827
DOI:10.1021/la1037712