Molecular determinant-based typing of KIR alleles and KIR ligands

Bibliographische Detailangaben
Veröffentlicht in:	Clinical immunology (Orlando, Fla.). - 1999. - 138(2011), 3 vom: 03. März, Seite 274-81
1. Verfasser:	Bari, Rafijul (VerfasserIn)
Weitere Verfasser:	Leung, Matthias, Turner, Victoria E, Embrey, Christy, Rooney, Barbara, Holladay, Martha, Leung, Wing
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2011
Zugriff auf das übergeordnete Werk:	Clinical immunology (Orlando, Fla.)
Schlagworte:	Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't HLA-A Antigens HLA-B Antigens HLA-C Antigens KIR2DL1 protein, human Ligands Receptors, KIR2DL1

Beschreibung
Zusammenfassung:	Copyright © 2010 Elsevier Inc. All rights reserved. Killer cell immunoglobulin-like receptors (KIRs) regulate NK cell function. KIRs and their HLA ligands are highly polymorphic in nature with substantial allelic polymorphism. At present, there is a lack of an expedient method for KIR and HLA allele typing with relevant functional information. Here, we developed a single-nucleotide polymorphism (SNP) assay to type various allele groups of KIR2DL1 with distinct functional properties based on polymorphism at position 245. We also established a SNP assay to type different KIR ligands based on polymorphism at position 77 in HLA-C and position 83 in HLA-B and -A. Our SNP assays for KIR and KIR ligand typing are much cheaper and faster than existing high-resolution typing. Importantly, our high-throughput methods provide readouts that are informative in predicting NK cell activity in health, disease, and transplantation
Beschreibung:	Date Completed 14.04.2011 Date Revised 20.10.2021 published: Print-Electronic Citation Status MEDLINE
ISSN:	1521-7035
DOI:	10.1016/j.clim.2010.12.002