Both HY5 and HYH are necessary regulators for low temperature-induced anthocyanin accumulation in Arabidopsis seedlings

Copyright © 2010 Elsevier GmbH. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 168(2011), 4 vom: 01. März, Seite 367-74
1. Verfasser: Zhang, Yongqiang (VerfasserIn)
Weitere Verfasser: Zheng, Sheng, Liu, Zhongjuan, Wang, Liguang, Bi, Yurong
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2011
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Anthocyanins Arabidopsis Proteins Basic-Leucine Zipper Transcription Factors Carrier Proteins DNA-Binding Proteins HY5 protein, Arabidopsis HYH protein, Arabidopsis Nuclear Proteins
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520 |a The roles of two bZIP transcription factors LONG HYPOCOTYL 5 (HY5) and HY5 HOMOLOG (HYH) in inducing anthocyanin accumulation during low temperature treatment were studied in Arabidopsis. In all seedlings tested, low temperature significantly induced anthocyanin accumulation only in the presence of light. In the absence of HY5 or HYH, the low temperature-induced anthocyanin accumulation was significantly impaired compared to that of the wild type. Moreover, in the double mutant hy5hyh, no significant anthocyanin accumulation was induced by low temperature even in light, suggesting that the low temperature-induced anthocyanin accumulation was mediated by HY5/HYH. Through the RT-PCR assay, expressions of several "early" genes in anthocyanin biosynthetic pathway, chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), were up-regulated by low temperature in a manner that is at most partially dependent on HY5/HYH, whereas dihydroflavanol reductase (DFR), a "late" gene, was found to be up-regulated in a manner that was almost fully dependent on HY5/HYH. Thus, up-regulation of DFR in a HY5/HYH-dependent manner may address the question of why low temperature-induced anthocyanin accumulation relies upon light. In addition, we found that HY5/HYH expression was enhanced by low temperature in wild type Col-0, implying that low temperature induces anthocyanin accumulation, at least in part, through enhancing HY5/HYH protein levels. Collectively, our data suggest that HY5 and HYH are two necessary regulators that play a pivotal role during low temperature-induced anthocyanin accumulation in Arabidopsis seedlings 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Anthocyanins  |2 NLM 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a Basic-Leucine Zipper Transcription Factors  |2 NLM 
650 7 |a Carrier Proteins  |2 NLM 
650 7 |a DNA-Binding Proteins  |2 NLM 
650 7 |a HY5 protein, Arabidopsis  |2 NLM 
650 7 |a HYH protein, Arabidopsis  |2 NLM 
650 7 |a Nuclear Proteins  |2 NLM 
700 1 |a Zheng, Sheng  |e verfasserin  |4 aut 
700 1 |a Liu, Zhongjuan  |e verfasserin  |4 aut 
700 1 |a Wang, Liguang  |e verfasserin  |4 aut 
700 1 |a Bi, Yurong  |e verfasserin  |4 aut 
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